Although the conventional packed column has excellent selectivity and longer column life, it can obtain better peak asymmetry when the sample is acidic, neutral and weakly alkaline. However, when analyzing compounds with strong alkalinity, the peak shape of samples may become worse and the trailing tail is serious, leading to the decline of quantitative analysis accuracy. This phenomenon is caused by the residual silica-hydroxyl group in the fixed phase. Although many manufacturers made a second reaction on the surface of silica gel, that is, the so-called “sealing the tail”, in order to reduce the role of residual silicon hydroxyl, but often can not completely eliminate the residual silicon hydroxyl. Thermo’s Hypersil BDS (Base Deactived Silica) series, which limits the silicon hydroxyl left, can be used to create truly homogeneous, reversed-phase fillers specially suitable for basic compounds using modern derivative reaction technology
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